原创论文
2021-10-28
Chromatography columns and systems are critical factors to the successful downstream purification of valuable biopharmaceutical molecules. Empty columns are available from several suppliers in standard and customized formats from lab scale to pilot and large scale production. They can accommodate multiple media types, when used appropriately, can maximizes process yields, boosts productivity, and shortens time-to-market.
Bio-Rad Process-Scale Chromatography Columns
Bio-Rad offers process-scale chromatography columns. Featured products include: Bio-Rad® EasyPack™ Columns have variable bed-height adaptors, inflatable seals, and fixed-bottom endpieces. Bio-Rad® InPlace™ Columns are designed for industrial applications and allow contained filling, packing, unpacking, and cleaning in place. Customized Process Columns are specifically designed with customers to provide an affordable and timely solution.
Spectrum Labs Chromatography
Spectrum Chromatography manufactures laboratory instruments and glassware. Products include the Glenco® gas/liquid syringes, glass chromatography columns, and Spectra/Chrom® instruments. Spectrum Chrom® Chromatography is the premier supplier of low-pressure liquid chromatography columns to biochemical researchers. The column bodies are borosilicate glass (generally Pyrex®) and the endplates are made of various plastics. Aqueous Series Columns feature black polypropylene endplates, and Organic Series Columns feature PTFE endplates, the only wetted materials in these columns are glass and inert fluoropolymer (PTFE). Spectrum’s disposable minicolumns are manufactured of ultrapure polystyrene and polypropylene. They have wide brimmed reservoirs to make packing quick and easy. The bed support is available in 3 different porosities to accommodate a variety of media. Spectra/Chrom® Column Support Hardware are availbe to support any of Spectrum columns.
GE Empty Columns for Bioprocess Manufacturing Scale
GE Healthcare Empty Column Products for Bioprocess Manufacturing Scale include AxiChrom 50 to 200 Columns, AxiChrom 300 to 1600 Columns, BPG Columns, Chromaflow Columns, ChromXACT Columns, FineLine Columns, and INdEX Columns.
Millipore Chromatography Hardware
QuikScale® chromatography columns are designed for scalability and rapid purification. It accommodates all media types, is easily and uniformly packed and delivers optimal resolution and product purity at faster linear velocities. IsoPak Chromatography Process columns are ideal for process scale chromatography, it has automated packing and unpacking features, slurry is pumped directly into the column through unique valve, enabling the column to be packed and unpacked without the removal of the top flow cell. Vantage® chromatography columns are ideal for development, pilot or production purification of pharmaceutical and biotechnology purification applications. Vantage columns are designed for simple assembly, scalability, and zero dead space. Vantage® Columns include Vantage® L Columns for small-particle media applications, Vantage® A2 Columns for use with rigid, higher-resolution chromatography media; and Vantage® S2 Columns for a general range of applications.
SEPRAGE Chromatography Columns
Sepragen’s Superflo and Wedge columns feature high performance at low pressures, high flow-rates, and small footprint, they help overcome the problems faced by bioprocess engineers and scientists as they scale up chromatographic processes from the laboratory to production scale. Click to read more comparison of Superflo Radial Flow Columns with other options.
Rhobust® EBA columns are designed to be free-flowing, The Rotating Fluid Distribution system create a sweeping motion along the entire cross- section of the column, helps fluid distribution and reduce risk of clogging. Learn More.
Pall Chromatography Columns for Process Development and Optimization
Pall Resolute FM columns are ideal for chromatographic purification from process development to manufacturing scale with a wide range of commonly used media. These glass and acrylic columns for development and manufacturing applications, are robust and incorporate new features to improve the outcome of column packing events. Resolute Chromatography Columns are suited for process purification in the biopharmaceutical industry. Three-position nozzle valves provide all column functions required for packing, unpacking and running of the column in a closed system.
ThermoFisher Scientific Custom Chromatography Services
ThermoFisher Scientific Custom Chromatography Services provide product and process specific affinity solutions such as Custom ligand development ›, Custom resin development ›, and Custom column packing ›
ABT Agrose Bead Technologies Empty Columns
ABT offers single-use chromatography tools to simplify the use of the resins. These accessories are convenient tools for manipulating small volumes of resins and the selection will depend on the equipment available in the purification step and the quantity of sample that needs to be purified. Featured products include Empty Mini Columns , Empty Spin Columns ,Empty Columns ,Empty Cartridges ,Empty FPLC Columns ,Empty Acrylic Column ,Empty Syringe Cartridges ,Flexi-column™.
Bio-Safe™ columns are “biocompatible” and precisionmachined from virgin PEEK™ (polyetheretherketone), Learn More from Triskem Bio-Safe™ Systems Document. Triskem Also offers Accessories for Columns and Cartridges.
Thomson Instrument Company Empty Columns
Single StEP Empty Columns (patented) provide different sized columns with a variety of sorbents and resins for purification applications, with multiple sizes for different scale. Single StEP® empty columns allow for the simple connection to FPLC/LPLC/MPLC systems.
References:
Column Packing for Process Scale Chromatography: Guidelines for Reproducibility
Chromatography Sample Integrity with High-Density Sample Handling Solutions
Biotechnology and Biopharmaceutical Manufacturing, Processing, and Preservation
Use of Sodium Hydroxide for Cleaning and Sanitizing Chromatography Media and Systems
Preparative chromatographic separation: Simulated moving bed and modified chromatography methods
General methods for flash chromatography using disposable columns
